Work

In Vivo Tomographic Imaging of Near-Infrared Fluorescent Probes

Year: 2002

Type: article

Abstract: Fluorescence imaging is increasingly used to probe protein function and gene expression in live animals. This technology could enhance the study of pathogenesis, drug development, and therapeutic intervention. In this article, we focus on three-dimensional fluorescence observations using fluorescence-mediated molecular tomography (FMT), a novel imaging technique that can resolve molecular function in deep tissues by reconstructing fluorescent probe distributions in vivo. We have compared FMT findings with conventional fluorescence reflectance imaging (FRI) to study protease function in nude mice with subsurface implanted tumors. This validation of FMT with FRI demonstrated the spatial congruence of fluorochrome activation as determined by the two techniques.

Source: Molecular Imaging

DOI: 10.1162/15353500200201121

Authors: Vasilis Ntziachristos, Christoph Bremer, Edward E. Graves, Jorge Ripoll, Ralph Weissleder

Institutions: Harvard University, Massachusetts General Hospital

Language: English

Topic: Optical Imaging and Spectroscopy Techniques

Subfield: Radiology, Nuclear Medicine and Imaging

Field: Medicine

Domain: Health Sciences

Open Access status: gold

Estimated APC paid: $2,175

Funder: National Cancer Institute