Work

Stimulation of the Drosophila immune system alters genome-wide nucleosome occupancy

Year: 2015

Type: article

Abstract: In eukaryotes, nucleosomes participate in all DNA-templated events by regulating access to the underlying DNA sequence. However, nucleosome dynamics during a genome response have not been well characterized [1,2]. We stimulated Drosophila S2 cells with heat-killed Gram-negative bacteria Salmonella typhimurium, and mapped genome-wide nucleosome occupancy at high temporal resolution by MNase-seq using Illumina HiSeq 2500. We show widespread nucleosome occupancy change in S2 cells during the immune response, with the significant nucleosomal loss occurring at 4 h after stimulation. Data have been deposited to the Gene Expression Omnibus (GEO) database repository with the dataset identifier GSE64507.

Source: Genomics Data

DOI: 10.1016/j.gdata.2015.01.001

Authors: Yingxue Ren, Daniel L. Vera, Kimberly A. Hughes, Jonathan H. Dennis

Institution: Florida State University

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $500

Funders: Florida State University, National Institute on Drug Abuse