Nedd5, a mammalian septin, is a novel cytoskeletal component interacting with actin-based structures.
Year: 1997
Type: preprint
Abstract: The mouse Nedd5 gene encodes a 41.5-kD GTPase similar to the Saccharomyces and Drosophila septins essential for cytokinesis. Nedd5 accumulates near the contractile ring from anaphase through telophase, and finally condenses into the midbody. Microinjection of anti-Nedd5 antibody interferes with cytokinesis, giving rise to binucleated cells. In interphase and postmitotic cells, Nedd5 localizes to fibrous or granular structures depending on the growth state of the cell. The Nedd5-containing fibers are disrupted by microinjection of GTPgammaS and by Nedd5 mutants lacking GTP-binding activity, implying that GTP hydrolysis is required for its assembly. The Nedd5-containing fibers also appear to physically contact actin bundles and focal adhesion complexes and are disrupted by cytochalasin D, C3 exoenzyme, and serum starvation, suggesting a functional interaction with the actin-based cytoskeletal systems in interphase cells.
Source: Genes & Development
Authors: M Kinoshita, S Kumar, A Mizoguchi, C Ide, A Kinoshita, T Haraguchi, Y Hiraoka, M Noda
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: bronze
Funders: Wellcome Trust, RIKEN
OpenAlex ID: W2129759722