Staphylococcus Aureus Infection Induces an Accumulation of Dibromotyrosine in the Thickened Superficial Layer of Chronic Cutaneous Wounds
Year: 2012
Type: article
Study design: Observational Study
Abstract: Chronic wounds are often infected with Staphylococcus aureus. Such an infection causes deterioration of tissue repair mechanisms. Wounds are composed of granulation tissue and a superficial cover layer consisting of necrotic tissue and inflammatory cell infiltrates. In the present study, we examined 30 patients with skin wounds, with or without Staphylococcus aureus infection. The patients were divided into 3 groups according to the number of Staphylococcus aureus at 10 5 CFU/g: lower value group (SA-I) , and higher value group (SA-II) . The thickness of the superficial layer and Staphylococcus aureus invasion were morphometrically analyzed. Staphylococcus aureus localized mostly in the superficial layer. The superficial layer becomes thicker in accordance with the increase in the number of Staphylococcus aureus. Patients in the SA -II group exhibit a significantly thicker superficial layer than those in SA-I group (p = 0.0001) ; however, no difference was found between the control and Staphylococcus aureus -I groups. Furthermore, the distance of Staphylococcus aureus invasion, measured histologically, was also significantly greater in SA-II than I (p = 0.003) . In the superficial layer, myeloperoxidase (MPO) -expressing neutrophils and MPO producing dibromotyrosine (DiBrY) , an oxidative product, were increasingly accumulated in accordance with the number of Staphylococcus aureus in the wound. These results suggest that DiBrY accumulation, which contributes to superficial layer thickening, is caused by the interaction between the infecting Staphylococcus aureus and the MPO-expressing activated neutrophils, which may form a biofilm in vivo. These events create poor conditions for tissue repair in chronic wounds.
Authors: Tsuyoshi Manabe, Shiro Jimi, Hiroshi Iwasaki, Hiroyuki Ohjimi
Language: English
Topic: Wound Healing and Treatments
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2130327653