IL-10-differentiated dendritic cells treatment for Experimental Autoimmune Encephalomyelitis (EAE), a model of human Multiple Sclerosis
Year: 2010
Type: dissertation
Abstract: Multiple sclerosis is a chronic autoimmune neurological disease characterized by inflammatory cell infiltration and demyelination in the central nervous system (CNS).It is considered to be mediated by Th1 and Th17 immune responses.Experimental autoimmune encephalomyelitis (EAE) is widely used as a mouse model to study MS as it has features and histopathology similar to that of MS.Tolerogenic dendritic cells (DC) are reported to efficiently prevent sensitization for EAE.In this research, we induced tolerogenic DC (DC10) by differentiating them with IL-10.Compared to immature DC, DC10 did not show increased expression of MHC II or the co-stimulatory molecules CD40, CD80 and CD86, and produced low levels of pro-inflammatory cytokines IL-1β, IL-6, and IL-12 but higher levels of IL-10.This is consistent with their possessing a tolerogenic phenotype.We found that three intraperitoneal (i.p.) injections of DC10 successfully inhibited the signs of established, ongoing EAE: DC10 significantly reduced the clinical scores, demyelination and cell infiltration in the spinal cord, as well as the production of IL-4, IL-6, IL-10, IL-17 and IFN-γ by spleen and lymph node (LN) lymphocytes.DC10 treatments did not significantly affect inflammatory cytokine mRNA levels in the CNS.We found that there was higher FoxP3 expression in the CNS in response to DC10 treatments relative to PBS-treated
Author: Siyuan Xie
Language: English
Subfield: Clinical Neurology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2130345361