Work

Generation of a chemical gradient across an array of 256 cell cultures in a single chip

Year: 2013

Type: article

Abstract: A microfluidic diffusion diluter to create stable chemical gradients across an array of cell cultures was demonstrated. The device enabled concentration based studies to be conducted at 256 different concentrations across individual, low shear cell cultures. A gradient of staurosporine on cells stained with Mitotracker Deep Red (MTDR) showed a concentration-based effect on cell apoptosis across the cell culture array.

Source: The Analyst

DOI: 10.1039/c3an00946g

Authors: Himali Somaweera, Akif Ibragimov, Dimitri Pappas

Institution: Texas Tech University

Language: English

Topic: Microfluidic and Capillary Electrophoresis Applications

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: green

Funders: National Institute of General Medical Sciences, National Center for Research Resources

Awards: G2519768640, G2590712157, Rapid Single Molecule Detection System for Intracellular Protease Activity, Rapid Single Molecule Detection System for Intracellular Protease Activity