Work

Cryo‐EM model validation using independent map reconstructions

Year: 2013

Type: article

Abstract: An increasing number of cryo-electron microscopy (cryo-EM) density maps are being generated with suitable resolution to trace the protein backbone and guide sidechain placement. Generating and evaluating atomic models based on such maps would be greatly facilitated by independent validation metrics for assessing the fit of the models to the data. We describe such a metric based on the fit of atomic models with independent test maps from single particle reconstructions not used in model refinement. The metric provides a means to determine the proper balance between the fit to the density and model energy and stereochemistry during refinement, and is likely to be useful in determining values of model building and refinement metaparameters quite generally.

Source: Protein Science

DOI: 10.1002/pro.2267

Authors: Frank DiMaio, Junjie Zhang, Wah Chiu, David Baker

Institutions: University of Washington, Stanford Medicine, Stanford University, Baylor College of Medicine

Language: English

Topic: Advanced Electron Microscopy Techniques and Applications

Subfield: Structural Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: National Institutes of Health, National Eye Institute, National Institute of General Medical Sciences

Awards: G1913224096, G2788500095, G380482681, Center for Protein Folding Machinery, 3 D Electron Microscopy of Macromolecules