Work

Construction of recombination-deficient strains of Streptococcus gordonii by disruption of the recA gene

Year: 1993

Type: article

Abstract: Degenerate oligonucleotide primers were used in a polymerase chain reaction (PCR) to amplify a region of the recA sequence of Streptococcus gordonii Challis. The resulting PCR fragment was cloned into the suicide vector pAM6199 and introduced into strain Challis, giving rise to recombination-deficient strains in which the recA gene was specifically inactivated.

Source: Journal of Bacteriology

DOI: 10.1128/jb.175.19.6354-6357.1993

Authors: M M Vickerman, D G Heath, D B Clewell

Institution: University of Michigan

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institute of Allergy and Infectious Diseases, National Institute of Dental and Craniofacial Research