Work

Interaction between relaxase MbeA and accessory protein MbeC of the conjugally mobilizable plasmid ColE1

Year: 2012

Type: article

Abstract: MbeA and MbeC are two key proteins in plasmid ColE1 conjugal mobilization. Isothermal titration calorimetry was used to detect and quantify an interaction between MbeA and MbeC. As a result of this interaction, the affinity of MbeA for single stranded DNA increased. The interaction was confirmed in vivo using a bacterial two-hybrid system, which revealed that MbeA-MbeC complexes are formed through the amino-terminal region of MbeA and the carboxy-terminal region of MbeC. To the best of our knowledge, this is the first report of direct interactions between conjugative proteins encoded by a mobilizable plasmid.

Source: FEBS Letters

DOI: 10.1016/j.febslet.2012.01.060

Authors: Athanasia Varsaki, Heather K. Lamb, Olga Eleftheriadou, Elpiniki Vandera, Paul Thompson, Gabriel Moncalián, Fernando de la Cruz, Alastair R. Hawkins, Constantin Drainas

Institutions: University of Ioannina, Newcastle University, Universidad de Cantabria

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funder: Newcastle University

OpenAlex ID: W2132062603

FWCI: 0.575

Cites: 34

Cited by: 15

Related to: 10