Work

Molecular cloning and sequencing of the gene encoding the fimbrial subunit protein of Bacteroides gingivalis

Year: 1988

Type: article

Abstract: The gene encoding the fimbrial subunit protein of Bacteroides gingivalis 381, fimbrilin, has been cloned and sequenced. The gene was present as a single copy on the bacterial chromosome, and the codon usage in the gene conformed closely to that expected for an abundant protein. The predicted size of the mature protein was 35,924 daltons, and the secretory form may have had a 10-amino-acid, hydrophilic leader sequence similar to the leader sequences of the MePhe fimbriae family. The protein sequence had no marked similarity to known fimbrial sequences, and no homologous sequences could be found in other black-pigmented Bacteroides species, suggesting that fimbrillin represents a class of fimbrial subunit protein of limited distribution.

Source: Journal of Bacteriology

DOI: 10.1128/jb.170.4.1658-1665.1988

Authors: D P Dickinson, M A Kubiniec, F Yoshimura, R J Genco

Institution: University at Buffalo, State University of New York

Language: English

Topic: Biochemical and Structural Characterization

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: American College of Dentists, National Institutes of Health, National Institute of Dental and Craniofacial Research

Awards: G2222376686, G605898261, G9000088753