Work

Simultaneous two-photon imaging and photo-stimulation with structured light illumination

Year: 2010

Type: article

Abstract: Holographic microscopy is increasingly recognized as a promising tool for the study of the central nervous system. Here we present a "holographic module", a simple optical path that can be combined with commercial scanheads for simultaneous imaging and uncaging with structured two-photon light. The present microscope is coupled to two independently tunable lasers and has two principal configurations: holographic imaging combined with galvo-steered uncaging and holographic uncaging combined with conventional scanning imaging. We applied this flexible system for simultaneous two-photon imaging and photostimulation of neuronal cells with complex light patterns, opening new perspectives for the study of brain function in situ and in vivo.

Source: Optics Express

DOI: 10.1364/oe.18.018720

Authors: Marco Dal Maschio, Francesco Difato, Riccardo Beltramo, Axel Blau, Fabio Benfenati, Tommaso Fellin

Institution: Italian Institute of Technology

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,270

Funders: University of Glasgow, Ministero dell’Istruzione, dell’Università e della Ricerca