Work

The TSC1-TSC2 complex consists of multiple TSC1 and TSC2 subunits

Year: 2012

Type: article

Abstract: BACKGROUND: Mutations to the TSC1 and TSC2 genes cause the disease tuberous sclerosis complex. The TSC1 and TSC2 gene products form a protein complex that integrates multiple metabolic signals to regulate the activity of the target of rapamycin (TOR) complex 1 (TORC1) and thereby control cell growth. Here we investigate the quaternary structure of the TSC1-TSC2 complex by gel filtration and coimmunoprecipitation. RESULTS: TSC1 and TSC2 co-eluted in high molecular weight fractions by gel filtration. Coimmunoprecipitation of distinct tagged TSC1 and TSC2 isoforms demonstrated that TSC1-TSC2 complexes contain multiple TSC1 and TSC2 subunits. CONCLUSIONS: TSC1 and TSC2 interact to form large complexes containing multiple TSC1 and TSC2 subunits.

Source: BMC Biochemistry

DOI: 10.1186/1471-2091-13-18

Authors: Marianne Hoogeveen-Westerveld, Leontine van Unen, Ans van den Ouweland, Dicky Halley, Andre Hoogeveen, Mark Nellist

Institution: Erasmus MC

Language: English

Topic: Tuberous Sclerosis Complex Research

Subfield: Genetics (clinical)

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $2,170