DLX5 expression is monoallelic and Dlx5 is up‐regulated in the Mecp2‐null frontal cortex
Year: 2008
Type: article
Abstract: There has been a controversy as to whether DLX5 is imprinted in human brain and lymphoblastoid cell lines (LCLs), and whether expression of Dlx5 and Dlx6 (Dlx5/6 exist as a bigene cluster), is dysregulated in the Mecp2-null mouse frontal cortex. In the September issue of Am J Hum Genet. (Vol 81: 492–506, 2007), Schüle et al. published a paper entitled ‘DLX5 and DLX6 expression is bi-allelic and not modulated by Mecp2 deficiency.’ This study concluded that DLX5 was not imprinted in normal human LCLs nor in human brain, contradicting the conclusions of Okita et al. (Genomics 81:556–559, 2003) and Horike et al. (Nature Genetics 37:31–40, 2005). Schüle et al. also claimed that expression of Dlx5 and Dlx6 in mouse brain varies greatly among individual mice and is not necessarily up-regulated in the frontal cortex of Mecp2-null mice. These findings directly contradict those of Horike et al. We have now repeated and verified our previous experiments that are relevant to the points raised by Schüle et al. Further, we have also expanded our analyses to determine why Schüle et al., were not able to reproduce our findings. Here are our findings in response to their assertions.
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: green
Funder: National Cancer Institute
OpenAlex ID: W2133316170