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Fluorescence Polarization Based Nucleic Acid Testing for Rapid and Cost‐Effective Diagnosis of Infectious Disease

Year: 2015
Type: article
Abstract: A new nucleic acid detection method was developed for a rapid and cost-effective diagnosis of infectious disease. This approach relies on the three unique elements: 1) detection probes that regulate DNA polymerase activity in response to the complementary target DNA; 2) universal reporters conjugated with a single fluorophore; and 3) fluorescence polarization (FP) detection. As a proof-of-concept, the assay was used to detect and sub-type Salmonella bacteria with sensitivities down to a single bacterium in less than three hours.
DOI: 10.1002/chem.201502934
Language: English
Open Access status: green
Funders: National Research Foundation of Korea, National Institutes of Health, U.S. Public Health Service, National Heart, Lung, and Blood Institute, National Institute of Allergy and Infectious Diseases, National Institute of Biomedical Imaging and Bioengineering
Awards: Improving diagnostic capabilities for typhoid fever, G1098359285, Novel Clickdyes for Biomedical Sensing, G2066243351, G2814483322, G3352905596, MAGNETIC NANOSENSORS FOR BIOMEDICAL ANALYSES OF MICROVESICLES, Chip NMR biosensor for molecular analysis of cells
OpenAlex ID: W2133494980
FWCI: 1.244
Cites: 34
Cited by: 20
Related to: 10