Possible Causes of Ileal Injury in Two Models of Microbial Sepsis and Protective Effect of
Year: 2010
Type: article
Abstract: Background: Sepsis related-multiple organ dysfunction is as-sociated with ileum injury. We aimed to determine the causes of ileal injury in two models of microbial sepsis resulted from in-fection with Aeromonas hydrophila or its endotoxin. We also evaluated the protective effect of phytic acid. Methods: Thin sections of ileum from 60 Swiss male mice in control, bacteria-infected or lipopolysaccharides (LPS) and bacteria-infected or LPS-infected co-administered with phytic acid were subjected to histopathological and TdT-mediated dUTP nick-end labeling (TUNEL) assay for apoptotic cells detection while ultra thin sections were stained with uranyl acetate and lead citrate for cytological changes examination. Also, ileum images were exposed to the image analysis soft-ware to determine some related morphometric measures. Results: Necrosis and apoptosis were observed in ileum injury in both examined sepsis models. The ileum injury was more severe in LPS model. Phytic acid showed the ability to attenu-ate ileum injury in Aeromonas hydrophila and its endotoxin models of sepsis after four weeks administration where its supplementation significantly minimized the histopathological and cytological complications and morphometric alterations resulted from the injury. Conclusion: The protective effects of phytic acid may be caused by increased mucous secretion, decreased apoptotic index, attenuating the inflammatory and lymphocytic cells count or increasing the renewal of the crypt cells and villous epithelial cells proliferation.
Authors: Rasha Rashad Ahmed, Hossam Ebaid
Institution: Beni-Suef University
Language: English
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2133943626