Work

Gcn5 regulates the dissociation of SWI/SNF from chromatin by acetylation of Swi2/Snf2

Year: 2010

Type: preprint

Abstract: The positive link between the SWI/SNF and the Gcn5 histone acetyltransferase in transcriptional activation has been well described. Here we report an inhibitory role for Gcn5 in SWI/SNF targeting. We demonstrate that Gcn5-containing complexes directly acetylate the Snf2 subunit of the SWI/SNF complex in vitro, as well as in vivo. Moreover, the acetylation of Snf2 facilitates the dissociation of the SWI/SNF complex from acetylated histones, and reduces its association with promoters in vivo. These data reveal a novel mechanism by which Gcn5 modulates chromatin structure not only through the acetylation of histones, but also by directly acetylating Snf2.

Source: Genes & Development

DOI: 10.1101/gad.1979710

Authors: Jeong-Hoon Kim, Anita Saraf, Laurence Florens, Michael Washburn, Jerry L. Workman

Institutions: Stowers Institute for Medical Research, University of Kansas Medical Center

Language: English

Topic: Chromatin Remodeling and Cancer

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: National Institutes of Health, National Institute of General Medical Sciences

Awards: G3623779153, Mechanisms of Transcriptional Regulation in Chromatin, G8596772093