Work

Green Fluorescent Protein-Tagged Retroviral Envelope Protein for Analysis of Virus-Cell Interactions

Year: 2003

Type: article

Abstract: Fluorescent retroviral envelope (Env) proteins were developed for direct visualization of viral particles. By fusing the enhanced green fluorescent protein (eGFP) to the N terminus of the amphotropic 4070A envelope protein, extracellular presentation of eGFP was achieved. Viruses incorporated the modified Env protein and efficiently infected cells. We used the GFP-tagged viruses for staining retrovirus receptor-positive cells, thereby circumventing indirect labeling techniques. By generating cells which conditionally expressed the GFP-tagged Env protein, we could confirm an inverse correlation between retroviral Env expression and infectivity (superinfection). eGFP-tagged virus particles are suitable for monitoring the dynamics of virus-cell interactions.

Source: Journal of Virology

DOI: 10.1128/jvi.77.10.6070-6075.2003

Authors: Dirk Spitzer, Kurt E. J. Dittmar, Manfred Rohde, Hansjörg Hauser, Dagmar Wirth

Institution: Helmholtz Centre for Infection Research

Language: English

Topic: Virus-based gene therapy research

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2134612137

FWCI: 1.324

Cites: 11

Cited by: 15

Related to: 10