Work

Detection of Functional Ligand-Binding Events Using Synchrotron X-Ray Scattering

Year: 2007

Type: article

Abstract: Small-molecule ligands that change the structure of a protein are likely to affect its function, whereas those causing no structural change are less likely to be functional. Wide-angle x-ray scattering (WAXS) can be easily carried out on proteins and small molecules in solution in the absence of chemical tags or derivatives. The authors demonstrate that WAXS is a sensitive probe of ligand binding to proteins in solution and can distinguish between nonfunctional and productive binding. Furthermore, similar ligand-binding modes translate into similar scattering patterns. This approach has high potential as a novel, generic, low-throughput assay for functional ligand binding.

Source: SLAS DISCOVERY

DOI: 10.1177/1087057107306104

Authors: Diane J. Rodi, Suneeta Mandava, David B. Gore, Lee Makowski, Robert F. Fischetti

Institutions: Argonne National Laboratory, Illinois Institute of Technology, Advanced Photon Source

Language: English

Topic: Enzyme Structure and Function

Subfield: Materials Chemistry

Field: Materials Science

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $2,070

Funders: National Institutes of Health, National Center for Research Resources