Introduction of the Foreign Transposon Tn 4560 in Streptomyces coelicolor Leads to Genetic Instability near the Native Insertion Sequence IS 1649
Year: 2007
Type: article
Abstract: We report an altered pattern of genetic instability for Streptomyces coelicolor when the bacterium harbored a foreign transposon, Tn4560. Deletions, amplifications, and circularizations of the linear 8.7-Mb chromosome occurred more frequently at sites adjacent to native insertion elements, notably IS1649. In contrast, deletions, amplifications, and circularizations of a wild-type strain happened at heterogeneous sites within the chromosome. In 50 strains examined, structural changes removed or duplicated hundreds of contiguous S. coelicolor genes, altering up to 33% of the chromosome. S. coelicolor shows a bias toward one type of genetic instability during this particular assault from the environment, the invasion of foreign DNA.
Source: Journal of Bacteriology
DOI: 10.1128/jb.00983-07
Authors: Elizabeth M. Widenbrant, Camilla M. Kao
Institution: Stanford University
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2135212856