Work

DNA modification of live cell surface

Year: 2009

Type: article

Abstract: We report a novel approach for the attachment of DNA fragments to the surface of live cells. By using fluorescence microscopy and flow cytometry we demonstrated that our synthetic conjugates of fatty acid with oligonucleotides can be incorporated in plasma membrane and then hybridized with complementary sequences at the cell surface. Method permits to control amount of immobilized DNA on the cell surface. All procedures can be completed within minutes and do not alter cell viability. Using this approach we tethered floating myeloid HL-60 cells to adherent A431 epitheliocytes in a sequence specific fashion. Thus, this method allows rapid and simple DNA multicoding of the cell surface and, therefore, opens new opportunities in manipulating with cell-cell interactions.

Source: Nucleic Acids Research

DOI: 10.1093/nar/gkn1034

Authors: Grigory G. Borisenko, Marina A. Zaitseva, Andrey N. Chuvilin, Galina E. Pozmogova

Institutions: Russian Academy of Sciences, Federal Research and Clinical Center of Physical-Chemical Medicine named after Y.M. Lopukhin, Bioengineering Center

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $3,992

Funder: University of Oxford