Work

Simultaneous two-wavelength transmission quantitative phase microscopy with a color camera

Year: 2010

Type: article

Abstract: We present a quantitative phase microscopy method that uses a Bayer mosaic color camera to simultaneously acquire off-axis interferograms in transmission mode at two distinct wavelengths. Wrapped phase information is processed using a two-wavelength algorithm to extend the range of the optical path delay measurements that can be detected using a single temporal acquisition. We experimentally demonstrate this technique by acquiring the phase profiles of optically clear microstructures without 2pi ambiguities. In addition, the phase noise contribution arising from spectral channel crosstalk on the color camera is quantified.

Source: Optics Letters

DOI: 10.1364/ol.35.002612

Authors: Matthew T. Rinehart, Natan T. Shaked, Nathan J. Jenness, Robert L. Clark, Adam Wax

Institution: Duke University

Language: English

Topic: Digital Holography and Microscopy

Subfield: Atomic and Molecular Physics, and Optics

Field: Physics and Astronomy

Domain: Physical Sciences

Open Access status: green

Funder: National Science Foundation

Awards: NIRT: Hierarchical Bionanomanufacturing, Molecular imaging of live cells using hyperspectral darkfield microscopy