Work

A membrane-activatable near-infrared fluorescent probe with ultra-photostability for mitochondrial membrane potentials

Year: 2015

Type: article

Abstract: Mitochondrial membrane potential (MMP) is a frequently used indicator for mitochondrial function. Herein, we report a photostable near-infrared (NIR) fluorescent dye for monitoring MMP. This new probe, named NIMAP, is non-fluorescent in aqueous solution and can be activated by cell membranes, providing high fluorescence contrast and low background fluorescence. NIMAP has been validated for monitoring MMP in living mammalian cells and in mice. Due to the large fluorescence response, low fluorescence background, high photostability, and excellent tissue penetration resulting from red-shifted excitation and emission in the "optical window" above 600 nm, broad applications of this new probe are expected.

Source: The Analyst

DOI: 10.1039/c5an01860a

Authors: Wei Ren, Ao Ji, Omran Karmach, David G. Carter, Manuela M. Martins-Green, Hui-wang Ai

Institutions: University of California, Riverside, Institute for Integrative Genome Biology

Language: English

Topic: Molecular Sensors and Ion Detection

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funders: National Science Foundation, University of California, Riverside, Division of Chemistry

Awards: G3306217783, CAREER: Expanding the Toolbox of Encodable Fluorescent Probes to Image Macrophage Redox Dynamics, G5986892838, G6671297155, G7635620518