Work

Imaging engineered tissues using structural and functional optical coherence tomography

Year: 2009

Type: article

Abstract: As the field of tissue engineering evolves, there will be an increasingly important need to visualize and track the complex dynamic changes that occur within three-dimensional constructs. Optical coherence tomography (OCT), as an emerging imaging technology applied to biological materials, offers a number of significant advantages to visualize these changes. Structural OCT has been used to investigate the longitudinal development of engineered tissues and cell dynamics such as migration, proliferation, detachment, and cell-material interactions. Optical techniques that image functional parameters or integrate multiple imaging modalities to provide complementary contrast mechanisms have been developed, such as the integration of optical coherence microscopy with multiphoton microscopy to image structural and functional information from cells in engineered tissue, optical coherence elastography to generate images or maps of strain to reflect the spatially-dependent biomechanical properties, and spectroscopic OCT to differentiate different cell types. From these results, OCT demonstrates great promise for imaging and visualizing engineered tissues, and the complex cellular dynamics that directly affect their practical and clinical use.

Source: Journal of Biophotonics

DOI: 10.1002/jbio.200910048

Authors: Xing Liang, Benedikt W. Graf, Stephen A. Boppart

Institution: University of Illinois Urbana-Champaign

Language: English

Topic: Optical Coherence Tomography Applications

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: green

Funders: Nankai University, National Institutes of Health, University of Illinois at Urbana-Champaign, National Institute of Biomedical Imaging and Bioengineering

Awards: G2362271019, G3342837969