Work

Development of a Sensitive Gene Expression Reporter System and an Inducible Promoter-Repressor System for Clostridium acetobutylicum

Year: 2003

Type: article

Abstract: A sensitive gene expression reporter system was developed for Clostridium acetobutylicum ATCC 824 by using a customized gusA expression cassette. In discontinuous cultures, time course profiles of beta-glucuronidase specific activity reflected adequately in vivo dynamic up- and down-regulation of acidogenesis- and/or solventogenesis-associated promoter expression in C. acetobutylicum. Furthermore, a new inducible gene expression system was developed in C. acetobutylicum, based on the Staphylococcus xylosus xylose operon promoter-repressor regulatory system.

Source: Applied and Environmental Microbiology

DOI: 10.1128/aem.69.8.4985-4988.2003

Authors: Laurence Girbal, Isabelle Mortier-Barrière, Frédéric Raynaud, Céline Rouanet, Christian Croux, Philippe Soucaille

Institutions: Centre National de la Recherche Scientifique, Institut National des Sciences Appliquées de Toulouse, Institut National de la Recherche Agronomique, Institut National des Sciences Appliquées de Lyon

Language: English

Topic: Microbial Metabolic Engineering and Bioproduction

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2136479223

FWCI: 0.377

Cites: 24

Cited by: 83

Related to: 10