Work

In vivo two-photon microscopy to 1.6-mm depth in mouse cortex

Year: 2011

Type: article

Abstract: Deep tissue in vivo two-photon fluorescence imaging of cortical vasculature in a mouse brain using 1280-nm excitation is presented. A record imaging depth of 1.6 mm in mouse cortex is achieved in vivo, approximately reaching the fundamental depth limit in scattering tissue.

Source: Journal of Biomedical Optics

DOI: 10.1117/1.3646209

Authors: Demirhan Kobat, Nicholas G. Horton, Chris Xu

Institution: Cornell University

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funder: National Science Foundation

Awards: GRADUATE RESEARCH FELLOWSHIP PROGRAM, CAREER: Instrument Development for Biological Imaging