Work

Roles for Non-TATA Core Promoter Sequences in Transcription and Factor Binding

Year: 2000

Type: article

Abstract: Sequence blocks within the core region were swapped among RNA polymerase II promoters to explore effects on transcription in vitro. The pair of blocks flanking TATA strongly influenced general transcription, with an additional effect on promoter activation. These flanking elements induced a change in the ratio of activated to basal transcription, whereas swapping TATA and initiator sequences only altered general transcription levels. Swapping the flanking blocks influenced binding by general transcription factors TBP and TFIIB. The results suggest that the architecture of the extended core sequence is important in determining promoter-specific effects on both general transcription levels and the tightness of regulation.

Source: Molecular and Cellular Biology

DOI: 10.1128/.20.10.3608-3615.2000

Authors: Branden S. Wolner, Jay D. Gralla

Institution: University of California, Los Angeles

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: U.S. Public Health Service, National Institute of General Medical Sciences

Awards: G237424500, G3766552459, Research Tranining in Cellular and Molecular Biology, Mechanism of Initiation by RNA polymerase II