Molecular Orientation Affects Localization Accuracy in Superresolution Far-Field Fluorescence Microscopy
Year: 2010
Type: article
Abstract: We investigate the cooperative effect of molecular tilt and defocus on fluorophore localization by centroid calculation in far-field superresolution microscopy based on stochastic single molecule switching. If tilt angle and defocus are unknown, the localization contains systematic errors up to about ±125 nm. When imaging rotation-impaired fluorophores of unknown random orientation, the average localization accuracy in three-dimensional samples is typically limited to about ±32 nm, restricting the attainable resolution accordingly.
Source: Nano Letters
DOI: 10.1021/nl103472b
Authors: Johann Engelhardt, Jan Keller, Patrick Hoyer, Matthias Reuss, Thorsten Staudt, Stefan W. Hell
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2136683606