Year: 2005
Type: dissertation
Abstract: TUMOR CELLS WITH TYROSINE KINASE GENE ALTERATIONS (I, I I ) .………….……. .………….……………. .4.1.1Shc associates with RET oncoproteins …………………………. . .4.1.2Identification of putative Shc docking sites on RET ……………. .4 . 1 .3 T y r 5 8 6 o f R E T / P T C 2 i s t h e d o c k i n g s i t e f o r S h c ………………. .4.1.4Asn583 of RET/PTC2 is required for high affinity binding of Shc 4.1.5Asn583 and Tyr586 are both required for transforming activity of R E T / P T C 2 o n c o g e n e …………………………………………………… 4.1.6Transforming activity of RET/PTC2 depends on activation of Shc 4.2 ALTERNATE SPLICING INFLUENCES RET SIGNALLING P OT E NT I AL ( I , I I I ) ……………………………………………………. .4.2.1 Tyr1096 functionally substitutes the Tyr1062 multidocking site in RET-d e p e n d e n t s c a t t e r i n g ……………………………………………… 4.2.2RET-dependent cell scattering and phosphorylation of Shc, Akt, E R K1 / 2 , a n d p 3 8 ………………………………………………………. .4.2.3Tyr1096 functionally substitutes the Tyr1062 in RET-dependent c e l l b r a n c h i n g …………………………………………………………. . .4.2.4RET-dependent branching of MDCK cells requires Grb2-coupled p a t h wa y s ………………………………………………………………. . .4.2.5 Tyr1062 is required for transforming activity of both RET i s o f o r ms ………………………………………………………………… 4.3 RET-DEPENDENT AND RET-INDEPENDENT SIGNALLING PATHWAYS IN TUMOR BIOLOGY AND DEVELOPMENTAL B I OL OGY ( I V, V) ……………………………………………………….4.3.1 RET kinase activity and PLC signalling are impaired by HSCR mu t a t i o n s a n d a c t i v a t e d b y ME N 2 mu t a t i o n s …………………………. .4.3.2Janus-C 6 2 0 R d i me r s a r e u n r e s p o n s i v e t o GDNF ………………… 4.3.3Janus-C620R and HSCR mutations impair motility and migration, epithelial tubule formation and protection from apoptosis of GDNF stimulated RET-e x p r e s s i n g c e l l s ………………………………. .4.3.4Janus-C620R and MEN 2A-C634R promote rapid cell p r o l i f e r a t i o n a n d f o r m c o l o n i e s i n s o f t a g a r ………………………….… 4.3.5 RET-independent morphological responses to GDNF: restoration of the renal phenotype of ret-deficient mice and induction of branching of gfr 1-MDC K c e l l s …………………………………………………. . .4.3.6GDNF activates Met in both RET-dependent and -independent s i g n a l l i n g mo s t l i k e l y b y S r c f a mi l y k i n a s e s ………………….………. . .
Source: Työväentutkimus Vuosikirja
Author: Elena Arighi
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2136769723