Work

A Rationally Designed Pyrrolysyl-tRNA Synthetase Mutant with a Broad Substrate Spectrum

Year: 2012

Type: article

Abstract: Together with tRNA(CUA)(Pyl), a rationally designed pyrrolysyl-tRNA synthetase mutant N346A/C348A has been successfully used for the genetic incorporation of a variety of phenylalanine derivatives with large para substituents into superfolder green fluorescent protein at an amber mutation site in Escherichia coli. This discovery greatly expands the genetically encoded noncanonical amino acid inventory and opens the gate for the genetic incorporation of other phenylalanine derivatives using engineered pyrrolysyl-tRNA synthetase-tRNA(CUA)(Pyl) pairs.

Source: Journal of the American Chemical Society

DOI: 10.1021/ja211972x

Authors: Yane-Shih Wang, Xinqiang Fang, Ashley L. Wallace, Bo Wu, Wenshe R. Liu

Institution: Texas A&M University

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Texas A and M University, Chongqing University of Arts and Sciences, National Cancer Institute

Award: Phage Display with Two Genetically Incorporated Noncanonical Amino Acids