Capillary electrophoresis–mass spectrometry as a powerful tool in biomarker discovery and clinical diagnosis: An update of recent developments
Year: 2008
Type: review
Abstract: Proteome analysis has emerged as a powerful technology to decipher biological processes. One of the main goals is to discover biomarkers for diseases from tissues and body fluids. However, the complexity and wide dynamic range of protein expression present an enormous challenge to separation technologies and mass spectrometry (MS). In this review, we examine the limitations of proteomics, and aim towards the definition of the current key prerequisites. We focus on capillary electrophoresis coupled to mass spectrometry (CE-MS), because this technique continues to show great promise. We discuss CE-MS from an application point of view, and evaluate its merits and vices for biomarker discovery and clinical applications. Finally, we present several examples on the use of CE-MS to determine urinary biomarkers and implications for disease diagnosis, prognosis, and therapy evaluation.
Source: Mass Spectrometry Reviews
DOI: 10.1002/mas.20205
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
Awards: Molecular Basis of Pathogenicity of IgA1-containing Immune Complexes, Sequential Ion/Ion Reactions for Large Peptide and Whole Protein Characterization, Mucosal HIV and Immunobiology Center, IgA-secreting B cell lines: a novel tool for studies of IgA nephropathy, Familial IgA Nephropathy: Genetic and Metabolic Studies, Cardiovascular Functional Genomics: translating experimental work to human disease., G6020720004, G6549036791, IgA1 glycosylation and receptor interactions in IgA nephropathy, Molecular Basis of Pathogenicity of IgA1-containing Immune Complexes, G944034983
OpenAlex ID: W2137274797