Involvement of the Saccharomyces cerevisiae Hydrolase Ldh1p in Lipid Homeostasis
Year: 2011
Type: article
Abstract: Here, we report the functional characterization of the newly identified lipid droplet hydrolase Ldh1p. Recombinant Ldh1p exhibits esterase and triacylglycerol lipase activities. Mutation of the serine in the hydrolase/lipase motif GXSXG completely abolished esterase activity. Ldh1p is required for the maintenance of a steady-state level of the nonpolar and polar lipids of lipid droplets. A characteristic feature of the Saccharomyces cerevisiae Δldh1 strain is the appearance of giant lipid droplets and an excessive accumulation of nonpolar lipids and phospholipids upon growth on medium containing oleic acid as a sole carbon source. Ldh1p is thought to play a role in maintaining the lipid homeostasis in yeast by regulating both phospholipid and nonpolar lipid levels.
Source: Eukaryotic Cell
DOI: 10.1128/ec.05040-11
Institutions: Ruhr University Bochum, Graz University of Technology
Language: English
Subfield: Biochemistry
Domain: Life Sciences
Open Access status: diamond
Funder: Deutsche Forschungsgemeinschaft
OpenAlex ID: W2137345000