Work

Production of Recombinant Adeno‐Associated Viral Vectors and Use in In Vitro and In Vivo Administration

Year: 2011

Type: article

Abstract: Adeno-associated virus is a nonpathogenic human virus that has been developed into a gene-delivery vector due to its high efficiency of infection for many different cell types and its ability to persist and lead to long-term gene expression. This unit describes efficient methods to generate high-titer, research-grade, adenovirus-free recombinant single-stranded and self-complementary adeno-associated virus in various serotypes, along with methods to quantify the viral vectors. Two detailed methods are provided for viral vector delivery into the rodent brain and spinal cord, and for histological detection of transgene expression of GFP.

Source: Current Protocols in Neuroscience

DOI: 10.1002/0471142301.ns0417s57

Authors: Steven J. Gray, Vivian W. Choi, Aravind Asokan, Rebecca A. Haberman, Thomas J. McCown, Richard Jude Samulski

Institution: University of North Carolina at Chapel Hill

Language: English

Topic: Virus-based gene therapy research

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institutes of Health, National Heart, Lung, and Blood Institute, National Institute of Allergy and Infectious Diseases, National Institute of Arthritis and Musculoskeletal and Skin Diseases