Work

Mutations in both the 2B and 2C genes of hepatitis A virus are involved in adaptation to growth in cell culture

Year: 1992

Type: article

Abstract: Oligonucleotide-directed mutagenesis of an infectious cDNA clone of wild-type hepatitis A virus was performed to determine which mutations acquired in the nonstructural 2B and 2C genes during adaptation to growth in cell culture were effective in enhancing virus growth in vitro. Results of transfection assays demonstrated that one mutation in the 2B gene and two mutations in the 2C gene were responsible for an increased efficiency in growth, but growth enhancement required the participation of at least two of the three mutations.

Source: Journal of Virology

DOI: 10.1128/jvi.66.2.650-654.1992

Authors: S U Emerson, Y K Huang, C McRill, M Lewis, R H Purcell

Institution: National Institute of Allergy and Infectious Diseases

Language: English

Topic: Hepatitis Viruses Studies and Epidemiology

Subfield: Hepatology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: bronze

OpenAlex ID: W2138152888