A Multienzyme Complex Channels Substrates and Electrons through Acetyl-CoA and Methane Biosynthesis Pathways in Methanosarcina
Year: 2014
Type: article
Abstract: Multienzyme complexes catalyze important metabolic reactions in many organisms, but little is known about the complexes involved in biological methane production (methanogenesis). A crosslinking-mass spectrometry (XL-MS) strategy was employed to identify proteins associated with coenzyme M-coenzyme B heterodisulfide reductase (Hdr), an essential enzyme in all methane-producing archaea (methanogens). In Methanosarcina acetivorans, Hdr forms a multienzyme complex with acetyl-CoA decarbonylase synthase (ACDS), and F420-dependent methylene-H4MPT reductase (Mer). ACDS is essential for production of acetyl-CoA during growth on methanol, or for methanogenesis from acetate, whereas Mer is essential for methanogenesis from all substrates. Existence of a Hdr:ACDS:Mer complex is consistent with growth phenotypes of ACDS and Mer mutant strains in which the complex samples the redox status of electron carriers and directs carbon flux to acetyl-CoA or methanogenesis. We propose the Hdr:ACDS:Mer complex comprises a special class of multienzyme redox complex which functions as a "biological router" that physically links methanogenesis and acetyl-CoA biosynthesis pathways.
Source: PLoS ONE
Language: English
Subfield: Building and Construction
Field: Engineering
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,382
Awards: GENETIC ANALYSIS OF HYDRO- GENOTROPHIC METHANOGENESIS BY METHANOSARCINA SPECIES, Redox Biology Center, Heterodisulfide Reductase in Methanosarcina acetivorans, G222768804, Redox Biology Center, GENETIC ANALYSIS OF HYDRO- GENOTROPHIC METHANOGENESIS BY METHANOSARCINA SPECIES, GENETIC ANALYSIS OF HYDROGENOTROPHIC METHANOGENESIS IN METHANOSARCINA SPECIES, G5478645508, G5648016710, G5681146612, G7824448954, EAGER: Coupling Electron Transport and Metabolism using Biological Routers
OpenAlex ID: W2138155770