Work

A fluorescent fatty acid probe, DAUDA, selectively displaces two myristates bound in human serum albumin

Year: 2011

Type: article

Abstract: 11-(Dansylamino) undecanoic acid (DAUDA) is a dansyl-type fluorophore and has widely used as a probe to determine the binding site for human serum albumin (HSA). Here, we reported that structure of HSA-Myristate-DAUDA ternary complex and identified clearly the presence of two DAUDA molecules at fatty acid (FA) binding site 6 and 7 of HSA, thus showing these two sites are weak FA binding sites. This result also show that DAUDA is an appropriate probe for FA site 6 and 7 on HSA as previous studied, but not a good probe of FA binding site 1 that is likely bilirubin binding site on HSA.

Source: Protein Science

DOI: 10.1002/pro.749

Authors: Yu Wang, Zhipu Luo, Xiaoli Shi, Huan Wang, Lei Nie, Mingdong Huang

Institutions: Chinese Academy of Sciences, Fujian Institute of Research on the Structure of Matter, State Key Laboratory of Structural Chemistry, Zhejiang Hisun Pharmaceutical (China)

Language: English

Topic: Protein Interaction Studies and Fluorescence Analysis

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: Salt Science Research Foundation, National Natural Science Foundation of China

Awards: G6314093954, 人血清白蛋白和药物以及药物－药物间的相互作用研究