Work

2D IRphoton echo of azido-probes for biomolecular dynamics

Year: 2010

Type: article

Abstract: The vibrations in the azido-, N(3), asymmetric stretching region of 2'-azido-2'-deoxyuridine (N(3)dU) are examined by two-dimensional infrared spectroscopy. In water and tetrahydrofuran (THF), the spectra display a single sharp diagonal peak that shows solvent sensitivity. The frequency-frequency correlation time in water is 1.5 ps, consistent with H-bond making and breaking dynamics. The 2D IR spectrum is reproduced for N(3)dU in water based on a model correlation function and known linear response functions. Its large extinction coefficient, vibrational frequency outside the protein and nucleic acid IR absorption, and sensitivity to water dynamics render -N(3) a very useful probe for 2D IR and other nonlinear IR studies: its signal is ca. 100 times that of nitriles.

Source: Physical Chemistry Chemical Physics

DOI: 10.1039/c0cp01625j

Authors: Matthew J. Tucker, Xin Sonia Gai, Edward E. Fenlon, Scott H. Brewer, Robin M. Hochstrasser

Institutions: University of Pennsylvania, Franklin & Marshall College

Language: English

Topic: Spectroscopy and Quantum Chemical Studies

Subfield: Atomic and Molecular Physics, and Optics

Field: Physics and Astronomy

Domain: Physical Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of General Medical Sciences, National Center for Research Resources

Awards: G255748793, Use of Vibrational Reporters to Measure Dynamics and Distances in RNA and Proteins, ULTRAFAST OPTICAL PROCESSES LABORATORY