Work

Isolation and Genotyping of Helicobacter pylori from Untreated Municipal Wastewater

Year: 2002

Type: article

Abstract: For this study, we isolated Helicobacter pylori from wastewater by a series of steps beginning with immunomagnetic separation and cell culture. After Gram staining and three standard microbial tests, the 16S rRNA sequences of a total of 23 out of 37 putative H. pylori isolates were verified by PCR. Eleven H. pylori isolates were genotyped and fell into four vacA classes: those with the vacA allelic variants s1a and m1, s1b and m1, s2 and m2, or s2 and m1. Most H. pylori isolates were of the vacA s1a/m1 type, which has been shown to be associated with advanced diseases based on genotyping of H. pylori from gastric cancer patients. These results demonstrated that H. pylori survives in water and may be a potential source of H. pylori transmission, especially where water is not adequately treated.

Source: Applied and Environmental Microbiology

DOI: 10.1128/aem.68.3.1436-1439.2002

Authors: Yingzhi Lu, Thomas E. Redlinger, Raquel Avitia, Adriana Galindo, Karen Goodman

Institution: The University of Texas at El Paso

Language: English

Topic: Helicobacter pylori-related gastroenterology studies

Subfield: Surgery

Field: Medicine

Domain: Health Sciences

SDGs: Clean water and sanitation, Good health and well-being

Open Access status: green

Funder: National Center for Research Resources