Work

The HIR corepressor complex binds to nucleosomes generating a distinct protein/DNA complex resistant to remodeling by SWI/SNF

Year: 2005

Type: preprint

Abstract: The histone regulatory (HIR) and histone promoter control (HPC) repressor proteins regulate three of the four histone gene loci during the Saccharomyces cerevisiae cell cycle. Here, we demonstrate that Hir1, Hir2, Hir3, and Hpc2 proteins form a stable HIR repressor complex. The HIR complex promotes histone deposition onto DNA in vitro and constitutes a novel nucleosome assembly complex. The HIR complex stably binds to DNA and nucleosomes. Furthermore, HIR complex binding to nucleosomes forms a distinct protein/DNA complex resistant to remodeling by SWI/SNF. Thus, the HIR complex is a novel nucleosome assembly complex which functions with SWI/SNF to regulate transcription.

Source: Genes & Development

DOI: 10.1101/gad.1341105

Authors: Philippe Prochasson, Laurence Florens, Selene K. Swanson, Michael P. Washburn, Jerry L. Workman

Institution: Stowers Institute for Medical Research

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: Leukemia and Lymphoma Society, National Institutes of Health, National Institute of General Medical Sciences

Awards: G3623779153, Mechanisms of Transcriptional Regulation in Chromatin