Protective Effects of Resveratrol on TNF- α -Induced Endothelial Cytotoxicity in Baboon Femoral Arterial Endothelial Cells

Year: 2013

Type: article

Abstract: Endothelial injury induced by inflammatory factors plays a critical role in the pathogenesis of cardiovascular disease. Endothelial cell (EC) apoptosis, proliferation, migration, and cellular adhesion molecule (CAM) expression contribute to the development of atherosclerosis. We investigated the effects of resveratrol (0.1-100 μ M) on the proliferation, migration, and CAM expression of primary cultures of baboon arterial endothelial cells (BAECs). In addition, we tested its effects under normal conditions as well as under inflammatory conditions induced by tumour necrosis factor-α (TNF-α) administered either by cotreatment, pretreatment, or posttreatment. Immunocytochemistry, MTT, wound-healing, and flow cytometry assays were performed. The resveratrol treatment significantly enhanced BAEC proliferation and attenuated TNF-α-induced impairment of proliferation at the optimal doses of 1-50 µM. Resveratrol at a high dose (100 μ M) and TNF-α impaired BAEC migration, while low doses of resveratrol (1-50 μ M) attenuated TNF-α-induced impairment of BAEC migration. Moreover, resveratrol inhibited TNF-α-induced ICAM-1 and VCAM-1 expression. Taken together, our results suggest that the resveratrol protects BAECs after inflammatory stimulation as well as ameliorates inflammatory effects at low concentrations. Consequently, resveratrol should be considered as a candidate drug for the prevention and treatment of inflammatory vascular diseases.

Source: Journal of Diabetes Research

DOI: 10.1155/2013/185172

Authors: Juan Xiao, Jun Song, Vida Hodara, Allen Ford, Xing Li Wang, Qiang Shi, Li Chen, John L. VandeBerg

Institutions: Texas Biomedical Research Institute, Shandong University, Qilu Hospital of Shandong University, Baylor College of Medicine

Language: English

Topic: Sirtuins and Resveratrol in Medicine

Subfield: Geriatrics and Gerontology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,760

Funders: National Natural Science Foundation of China, Natural Science Foundation of Shandong Province, National Institutes of Health, National Heart, Lung, and Blood Institute, Office of Research Infrastructure Programs, National Institutes of Health

OpenAlex ID: W2138948064