Work

Multiple Nucleosome Positioning Sites Regulate the CTCF-Mediated Insulator Function of the H19 Imprinting Control Region†

Year: 2002

Type: article

Abstract: The 5' region of the H19 gene harbors a methylation-sensitive chromatin insulator within an imprinting control region (ICR). Insertional mutagenesis in combination with episomal assays identified nucleosome positioning sequences (NPSs) that set the stage for the remarkably precise distribution of the four target sites for the chromatin insulator protein CTCF to nucleosome linker sequences in the H19 ICR. Changing positions of the NPSs resulted in loss of both CTCF target site occupancy and insulator function, suggesting that the NPSs optimize the fidelity of the insulator function. We propose that the NPSs ensure the fidelity of the repressed status of the maternal Igf2 allele during development by constitutively maintaining availability of the CTCF target sites.

Source: Molecular and Cellular Biology

DOI: 10.1128/mcb.22.10.3339-3344.2002

Authors: Meena Kanduri, Chandrasekhar Kanduri, Piero Mariano, Alexander A. Vostrov, Wolfgang Quitschke, Victor Lobanenkov, Rolf Ohlsson

Institutions: Uppsala University, State University of New York, Stony Brook University, National Institutes of Health, National Institute of Allergy and Infectious Diseases

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of Allergy and Infectious Diseases

OpenAlex ID: W2138952587

FWCI: 1.959

Cites: 23

Cited by: 72

Related to: 10