Work

All-plastic miniature fluorescence microscope for point-of-care readout of bead-based bioassays

Year: 2015

Type: article

Abstract: A number of new platforms have been developed for multiplexed bioassays that rely on imaging targeted fluorescent beads labeled with different fluorescent dyes. We developed a compact, low-cost three-dimensional printed fluorescence microscope that can be used as a detector for mutiplexed, bead-based assays to support point-of-care applications. Images obtained with the microscope were analyzed to differentiate multiple analytes in a single sample with a comparable limit of detection to commercially available macroscopic assay platforms.

Source: Journal of Biomedical Optics

DOI: 10.1117/1.jbo.20.10.105010

Authors: Alessandra Forcucci, Michal Emanuel Pawlowski, Zachary Crannell, Ina Pavlova, Rebecca Richards-Kortum, Tomasz S. Tkaczyk

Institution: Rice University

Language: English

Topic: Biosensors and Analytical Detection

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: bronze

Funder: Bill and Melinda Gates Foundation

Award: Rice University - to substantially reduce the cost of point-of-care diagnostic systems by developing a Readout & Signal Transduction component costing less than $10 ...