Work

Rebuttal: Adaptive Point Mutation (Rosenberg and Hastings)

Year: 2004

Type: article

Abstract: The Rosenberg-Hastings paper (4) defends a model much like that of Foster (1) and argues that the observed mutagen-esis contradicts predictions of the amplification model. On the contrary, amplification indirectly causes weak mutagenesis and allows that mutagenesis to have a detectable effect on lac reversion. However, this mutagenesis is a minor side effect that is neither sufficient nor necessary to explain reversion. Mu-tagenesis has distracted attention from the main message of this system—a target number increase during growth under selection. SELECTION INCREASES REVERTANT YIELD 104-FOLD Seen in the light of the amplification mutagenesis (AM) model, 100 lac revertant colonies arise from 106 plated cells that carry a lac duplication and can therefore grow under selection. This revertant frequency (104) is 104-fold higher

Source: Journal of Bacteriology

DOI: 10.1128/jb.186.15.4844.2004

Authors: John R. Roth, Dan I. Andersson

Institutions: University of California, Davis, Swedish Institute, Karolinska Institutet

Language: English

Topic: Evolution and Genetic Dynamics

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institutes of Health

Award: Regulation of an Error-Prone Polymerase