Work

Wnt-5/pipetail functions in vertebrate axis formation as a negative regulator of Wnt/β-catenin activity

Year: 2003

Type: article

Abstract: We provide genetic evidence defining a role for noncanonical Wnt function in vertebrate axis formation. In zebrafish, misexpression of Wnt-4, -5, and -11 stimulates calcium (Ca2+) release, defining the Wnt/Ca2+ class. We describe genetic interaction between two Wnt/Ca2+ members, Wnt-5 (pipetail) and Wnt-11 (silberblick), and a reduction of Ca2+ release in Wnt-5/pipetail. Embryos genetically depleted of both maternal and zygotic Wnt-5 product exhibit cell movement defects as well as hyperdorsalization and axis-duplication phenotypes. The dorsalized phenotypes result from increased beta-catenin accumulation and activation of downstream genes. The Wnt-5 loss-of-function defect is consistent with Ca2+ modulation having an antagonistic interaction with Wnt/beta-catenin signaling.

Source: The Journal of Cell Biology

DOI: 10.1083/jcb.200303107

Authors: Trudi A. Westfall, Ryan Brimeyer, Jen Twedt, Jean Gladon, Andrea Olberding, Makoto Furutani-Seiki, Diane C. Slusarski

Institution: University of Iowa

Language: English

Topic: Wnt/β-catenin signaling in development and cancer

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: March of Dimes Foundation, Holden Comprehensive Cancer Center, University of Iowa

OpenAlex ID: W2140328069

FWCI: 4.746

Cites: 58

Cited by: 276

Related to: 8