Work

Real‐Time SNP Analysis in Secondary‐Structure‐Folded Nucleic Acids

Year: 2010

Type: article

Abstract: A tricomponent sensor was designed for genotyping stem-loop-structured DNA sequences. DNA adaptor strand f (see picture; red) hybridizes to the analyte (orange) with high affinity and unwinds its secondary structure; strand m (green) forms a stable complex only with the fully complementary analyte sequence. A fluorescing molecular beacon reports complex formation.

Source: Angewandte Chemie International Edition

DOI: 10.1002/anie.201004475

Authors: Jeffrey Grimes, Yulia V. Gerasimova, Dmitry M. Kolpashchikov

Institution: University of Central Florida

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Human Genome Research Institute

Award: Nucleic acid analysis using deoxyribozyme technology