Work

Response to Comment on: Jowett et al. (2010) Genetic Variation at the FTO Locus Influences RBL2 Gene Expression. Diabetes;59:726–732

Year: 2010

Type: article

Abstract: The comment submitted by Berulava andHorsthemke (1) on our recent work regardingthe biological effects on gene expression of theFTO single nucleotide polymorphism (SNP) rs8050136 (2) is welcomed and achieves one of the study’s goals—to inspire follow-up studies on our observation that these SNPs may affect RBL2 expression but not FTO itself. The approach taken by Berulava and Horsthemke is to quantify allelic-specific transcript levels in cells that are heterozygous for a given indicator variant in the mRNA of the gene of interest (in this case, rs3929 in the 3 untrans-lated region of RBL2). They measure the ratio of allele-specific transcripts for RBL2 in three FTO SNP (rs8050136) heterozygotous individuals (AC) and two homozygous individuals (AA) and report that skewing of RBL2 allelic expression was detected but not significant. The presented data fail to replicate the observation published in our original study. There are many possible

Source: Diabetes

DOI: 10.2337/db10-0443

Authors: Jeremy B.M. Jowett, John Blangero

Institutions: Baker Heart and Diabetes Institute, Texas Biomedical Research Institute

Language: English

Topic: Genetic Associations and Epidemiology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

OpenAlex ID: W2140808846