Growth dynamics of gut microbiota in health and disease inferred from single metagenomic samples
Year: 2015
Type: article
Abstract: Metagenomic sequencing increased our understanding of the role of the microbiome in health and disease, yet it only provides a snapshot of a highly dynamic ecosystem. Here, we show that the pattern of metagenomic sequencing read coverage for different microbial genomes contains a single trough and a single peak, the latter coinciding with the bacterial origin of replication. Furthermore, the ratio of sequencing coverage between the peak and trough provides a quantitative measure of a species' growth rate. We demonstrate this in vitro and in vivo, under different growth conditions, and in complex bacterial communities. For several bacterial species, peak-to-trough coverage ratios, but not relative abundances, correlated with the manifestation of inflammatory bowel disease and type II diabetes.
Source: Science
Language: English
Topic: Gut microbiota and health
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Funders: Leona M. and Harry B. Helmsley Charitable Trust, Boehringer Ingelheim Fonds, Minerva Foundation, Ministry of Science, Technology and Space, Israel Science Foundation, Centre National de la Recherche Scientifique, Rising Tide Foundation, Benoziyo Endowment Fund for the Advancement of Science, National Institute of Diabetes and Digestive and Kidney Diseases, H2020 European Research Council
OpenAlex ID: W2141137078