Work

tRNA cleavage is a conserved response to oxidative stress in eukaryotes

Year: 2008

Type: article

Abstract: Recent results have identified a diversity of small RNAs in a wide range of organisms. In this work, we demonstrate that Saccharomyces cerevisiae contains a small RNA population consisting primarily of tRNA halves and rRNA fragments. Both 5' and 3' fragments of tRNAs are detectable by Northern blot analysis, suggesting a process of endonucleolytic cleavage. tRNA and rRNA fragment production in yeast is most pronounced during oxidative stress conditions, especially during entry into stationary phase. Similar tRNA fragments are also observed in human cell lines and in plants during oxidative stress. These results demonstrate that tRNA cleavage is a conserved aspect of the response to oxidative stress.

Source: RNA

DOI: 10.1261/rna.1232808

Authors: Debrah M. Thompson, Cheng Lu, Pamela J. Green, Roy Parker

Institutions: University of Arizona, Delaware Biotechnology Institute, University of Delaware, Howard Hughes Medical Institute

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Funders: National Science Foundation, Howard Hughes Medical Institute

Awards: Genome-wide Analysis of miRNAs and siRNAs associated with Abiotic Stress in Arabidopsis, Analysis of the Ribonucleases of Arabidopsis thaliana