Work

Antifungal Activity of Amphotericin B Cochleates against Candida albicans Infection in a Mouse Model

Year: 2000

Type: article

Abstract: Cochleates are lipid-based supramolecular assemblies composed of natural products, negatively charged phospholipid, and a divalent cation. Cochleates can encapsulate amphotericin B (AmB), an important antifungal drug. AmB cochleates (CAMB) have a unique shape and the ability to target AmB to fungi. The minimal inhibitory concentration and the minimum lethal concentration against Candida albicans are similar to that for desoxycholate AmB (DAMB; Fungizone). In vitro, CAMB induced no hemolysis of human red blood cells at concentrations of as high as 500 microg of AmB/ml, and DAMB was highly hemolytic at 10 microg of AmB/ml. CAMB protect ICR mice infected with C. albicans when the agent is administered intraperitoneally at doses of as low as 0.1 mg/kg/day. In a tissue burden study, CAMB, DAMB, and AmBisome (liposomal AmB; LAMB) were effective in the kidneys, but in the spleen CAMB was more potent than DAMB at 1 mg/kg/day and was equivalent to LAMB at 10 mg/kg/day. In summary, CAMB are highly effective in treating murine candidiasis and compare well with AmBisome and AmB.

Source: Antimicrobial Agents and Chemotherapy

DOI: 10.1128/aac.44.6.1463-1469.2000

Authors: Leila Zarif, John R. Graybill, David Perlin, Laura Najvar, Rosie Bocanegra, Raphael J. Mannino

Institutions: Rutgers New Jersey Medical School, The University of Texas at San Antonio Health Science Center

Language: English

Topic: Antifungal resistance and susceptibility

Subfield: Infectious Diseases

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institutes of Health, U.S. Public Health Service, National Institute of Allergy and Infectious Diseases