Effects of Cytochalasin B on the Response of Toad Urinary Bladder to Vasopressin
Year: 1974
Type: article
Abstract: A combined physiological and morphological study of the effects of cytochalasin B (CB) on the toad urinary bladder has been carried out. CB inhibits the hydro-osmotic response to vasopressin without altering basal water permeability or diffusion, or the increase in (3)H(2)O diffusion observed after hormone addition. Although CB increases [(22)Na]-, [(36)Cl]-, and [(14)C]urea fluxes, and decreases transepithelial potential, no alteration in basal short-circuit current, the vasopressin-induced increase in this parameter, or [(14)C]inulin permeability occurs. In the absence of hormone, CB does not markedly alter the structure of the toad bladder. However, in the presence of vasopressin, CB induces the formation of large intracellular vacuoles. These results suggest a possible coupling of solute and water movement across the tissue.
Source: The Journal of Cell Biology
DOI: 10.1083/jcb.63.3.986
Institutions: Baylor University Medical Center, University of Pennsylvania
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2141769942