Work

Label-free real-time imaging of myelination in the Xenopus laevis tadpole by in vivo stimulated Raman scattering microscopy

Year: 2014

Type: article

Abstract: The myelin sheath plays an important role as the axon in the functioning of the neural system, and myelin degradation is a hallmark pathology of multiple sclerosis and spinal cord injury. Electron microscopy, fluorescent microscopy, and magnetic resonance imaging are three major techniques used for myelin visualization. However, microscopic observation of myelin in living organisms remains a challenge. Using a newly developed stimulated Raman scattering microscopy approach, we report noninvasive, label-free, real-time in vivo imaging of myelination by a single-Schwann cell, maturation of a single node of Ranvier, and myelin degradation in the transparent body of the Xenopus laevis tadpole.

Source: Journal of Biomedical Optics

DOI: 10.1117/1.jbo.19.8.086005

Authors: Chun-Rui Hu, Delong Zhang, Mikhail N. Slipchenko, Ji-Xin Cheng, Bing Hu

Institutions: University of Science and Technology of China, Hefei National Center for Physical Sciences at Nanoscale, Purdue University West Lafayette

Language: English

Topic: Spectroscopy Techniques in Biomedical and Chemical Research

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: National Natural Science Foundation of China, National Institute of General Medical Sciences, National Institute of Biomedical Imaging and Bioengineering